Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing
نویسندگان
چکیده
منابع مشابه
Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers†
Cell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezing and the current state-of-the-art involves the addition of significant amounts of organic solven...
متن کاملSynthetic Non-Biodegradable Polymers:
Synthetic polymers have been widely used in making various medical devices, such as disposable supplies, implants, drug delivery systems and tissue engineering scaffolds. The advantages of using polymers, as biomaterials, are their manufacturability. Polymers are easy to fabricate into various sizes and shapes compared to metals and ceramics. They are also light in weight and with a wide range ...
متن کاملIce-Free Cryopreservation by Vitrification
Long-term preservation is crucial as an enabling technology for tissue banking for transplantation, regenerative medicine products that contain living cells and biopsy samples. Regenerative medicine reviews in particular have consistently highlighted the need for preservation methods for product development and commercialization. Strategic assessments of regenerative medicine have provided thre...
متن کاملIce Crystal Growth during the Rapid Freezing of Tissues
This paper contains a few comments on the physical analysis of the factors controlling the rate of cooling, and the relation of the rate of cooling to ice crystal formation which has been carried out in connection with the electron microscopic work described by Dr. C-ersh at this conference. Of the many factors which influence the rate of cooling, by far the most important is the size of the sa...
متن کاملPredehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation
Conventional approaches for cell cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved cells. Here, we show intentionally induced ice formation at a high subzero temperature (> -10 °C) during cryopreservation, which is often referred to as ice seeding, could result in significant cell injury in the abs...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2014
ISSN: 2041-1723
DOI: 10.1038/ncomms4244